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MOLECULAR CHARACTERIZATION AND EVALUATION OF AMYLASE PRODUCING BACTERIA FROM SOIL

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03 Pages : 12-27

http://dx.doi.org/10.31703/giidr.2023(VIII-I).03      10.31703/giidr.2023(VIII-I).03      Published : Dec 2023

Molecular Characterization and Evaluation of Amylase Producing Bacteria from Soil

Abstract

    In the current work, we used molecular approaches to collect and characterise bacteria that produce amylase from soil. Based on clear zones, three species were separated using iodine agar plates. Then, these bacterial isolates were characterised by 16S rRNA gene sequencing. Based on 16S rRNA analysis, the bacterial isolates were identified as Bacillus cerus, Bacillus subtilis, and Bacillus stratosphericus. In addition, numerous growth parameters were tuned to produce the most amylase possible. For both 24 and 48 hours, the isolates' maximum optical density for growth was seen at pH 7.0. Compared to nitrogen and peptone, all three of the bacterial isolates produced the greatest amount of amylase when maltose was utilised as the carbon source. Further investigation is needed to determine which novel isolates in this site have the highest amylase activity. Our findings indicate that this enzyme has a variety of potential industrial applications.

    Amylase, Optical Density, Enzyme Assay, Bacillus Cerus, Bacillus Subtilis, Bacillus Stratosphericus

    (1) Hira Ikram
    Lecturer, Institute of Biotechnology and microbiology, Bacha Khan University, Charsadda, KP, Pakistan.
    (2) Aiman Umer
    Assistant Professor, Department of Food and Nutrition, Shaheed Benazir Bhutto Women University, Peshawar, KP, Pakistan.
    (3) Muhammad Kashif
    Research officer, Institute of Soil and Environmental Sciences, University of Agriculture, Faisalabad, Punjab, Pakistan.
    (4) Bushra Rehman
    Assistant Professor, Institute of Biotechnology and Microbiology, Bacha Khan University, Charsadda, KP, Pakistan.
    (5) Numan Saleh Zada
    Senior Scientific Officer, Pakistan Council of Scientific and Industrial Research (PCSIR) Laboratories Complex, Peshawar, KP, Pakistan.
    (6) Sanam Islam Khan
    Senior Scientific Officer, Pakistan Council of Scientific and Industrial Research (PCSIR) Laboratories Complex, Peshawar, KP, Pakistan.
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Cite this article

APA

    APA : Ikram, H., Umer, A., & Kashif, M. (2023). Molecular Characterization and Evaluation of Amylase Producing Bacteria from Soil. Global Immunological & Infectious Diseases Review, VIII(I), 12-27. https://doi.org/10.31703/giidr.2023(VIII-I).03

CHICAGO

    CHICAGO : Ikram, Hira, Aiman Umer, and Muhammad Kashif. 2023. "Molecular Characterization and Evaluation of Amylase Producing Bacteria from Soil." Global Immunological & Infectious Diseases Review, VIII (I): 12-27 doi: 10.31703/giidr.2023(VIII-I).03

HARVARD

    HARVARD : IKRAM, H., UMER, A. & KASHIF, M. 2023. Molecular Characterization and Evaluation of Amylase Producing Bacteria from Soil. Global Immunological & Infectious Diseases Review, VIII, 12-27.

MHRA

    MHRA : Ikram, Hira, Aiman Umer, and Muhammad Kashif. 2023. "Molecular Characterization and Evaluation of Amylase Producing Bacteria from Soil." Global Immunological & Infectious Diseases Review, VIII: 12-27

MLA

    MLA : Ikram, Hira, Aiman Umer, and Muhammad Kashif. "Molecular Characterization and Evaluation of Amylase Producing Bacteria from Soil." Global Immunological & Infectious Diseases Review, VIII.I (2023): 12-27 Print.

OXFORD

    OXFORD : Ikram, Hira, Umer, Aiman, and Kashif, Muhammad (2023), "Molecular Characterization and Evaluation of Amylase Producing Bacteria from Soil", Global Immunological & Infectious Diseases Review, VIII (I), 12-27

TURABIAN

    TURABIAN : Ikram, Hira, Aiman Umer, and Muhammad Kashif. "Molecular Characterization and Evaluation of Amylase Producing Bacteria from Soil." Global Immunological & Infectious Diseases Review VIII, no. I (2023): 12-27. https://doi.org/10.31703/giidr.2023(VIII-I).03